%0 Journal Article
%T IMMOBILIZATION OF PENICILIN G ACYLASE ON POLYACRYLONITRILE FIBER<br>以聚丙烯腈纤维为载体制备固定化青霉素G酰化酶的研究
%A H Xian
%A Z Wang
%A <br>鲜海军
%A 王祯祥
%J 微生物学报
%D 2001
%I 
%X The immobilization of Penicillin G Acylase from Bacillus megaterium by glutaraldehyde crosslinking on the partially acid-hydrolyed polyacrylonitrile fiber was studied. When the amount of--NH2 on fiber were 690 mumol/g and the moisture in the fiber was 64%, and the content of enzyme protein immobilized on fiber was more than 100 mg/g. The activity of 2300 IU/g was obtained with 30% of overall yield and 56% of binding efficiency. The immobilization yield was markedly influenced by ratio of the amount of free enzyme used to the weight of the fiber. The half-life of storage stability of immobilized PGA at room temperature was 130 days. The immobilized PGA kept 80% of the initial activity after 20 cycles of operation in 10% of PGK(W/V) in 0.05 mol/L phosphate buffer, pH 8.0, at 37 degrees C and an enzyme load of 150 IU/g(PGK) and 10 g(PGK) for per cycle of operation. The hydrolysis conversion of PGK in the range of 2.5%-12.5% (W/V) were over 98% for the immobilized PGA. The operation stability of immobilized PGA treated with DTT was better than that of immobilized PGA untreated.
%K Penicillin G acylase
%K Immobilization of enzyme
%K Bacillus megaterium<br>青霉素酰化酶
%K 固定化酶
%K 巨大芽孢杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2A1C403E78BDF0D1&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=E158A972A605785F&sid=D93AD940782892D0&eid=03436AC72A659ACA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=14