%0 Journal Article
%T Optimization of liquid growth conditions and determination of growth curves for Bartonella species<br>巴尔通体液体培养条件简化及生长曲线观察
%A LI Dong-Mei
%A Miao Zhi-Gang
%A Song Xiu-Ping
%A Wang Jun
%A Liu Qi-Yong
%A <br>栗冬梅
%A 苗志刚
%A 宋秀平
%A 王君
%A 刘起勇
%J 微生物学通报
%D 2012
%I 
%X Objective] We tested an insect cells-based liquid medium and its utility for the easy-to-maintain, fast and reliable system for cultivation of different Bartonella. Methods] Media composition and growth conditions were optimized using B. henselae and B. quintana. The growth curves of the other ten Bartonella species were determined using the optimized liquid medium. Results] Glutamine and sucrose supplements did not affect significantly the growth rate of these bacteria. A generation time of 5.2 h for B. henselae and 4.3 h for B. quintana was estimated during the exponential phase of the growth curve occurred between days 1 and 2 after inoculation. The twelve medical and veterinary important Bartonella species grew well under conditions established and each exhibited unique growth characteristics. Conclusion] This liquid growth medium may provide an advantage over conventional direct blood agar plating for analyzing Bartonella properties including antibiotic susceptibility and resistance and pathogenicity.
%K Bartonella
%K Liquid culture
%K Growth curve
%K Cat scratch disease<br>巴尔通体
%K 液体培养
%K 生长曲线
%K 猫抓病
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=324F8504B2709856EE20D6919CDC2DB4&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=708DD6B15D2464E8&sid=4B35F43975EC564A&eid=FC8D65835446970D&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0