%0 Journal Article
%T Construction of Genetic Engineering Microogranism of Salt Tolerant Degrading Strain<br>耐盐及苯乙酸、甲基对硫磷降解基因工程菌的构建
%A Liu Zhi
%A Hong Qing
%A Xu Jianhong
%A Zhang Guoshun
%A Li Shunpeng
%A <br>刘智
%A 洪青
%A 徐剑宏
%A 张国顺
%A 李顺鹏
%J 微生物学报
%D 2003
%I 
%X H1 ( Halomonas sp.) was a strain of salt tolerance (18% NaCl, W/V) and degrading phenylacetic acid, plasmid pDT3 was a derivation of pUC19 inserted mpd gene (methyl parathion degrading gene), which is obtained from methyl parathion (MP) degrading strain DLL-E4 (Pseudomonas putida). Recombinant plasmids pKT-MP and pBBR-MP were constructed by inserting Hind III fragment (which include whole mpd gene) of pDT3 into broad host vector pKT230 and pBBR1-MCS2. With the aid of help-plasmid pRK2013, pKT-MP and pBBR-MP were transferred into H1 (called tri-parent conjugation) and multi-functioned GEMs H-pKT-MP and H-pBBR-MP were constructed, which were salt tolerant, phenylacetic acid-degrading and MP-degrading. The activity of MP hydrolase of H-pBBR-MP and H-pKT-MP was 1 x and 2 x the one of DLL-E4. The MP hydrolase activity of two GEMs were constant.
%K Salt tolerance
%K Phenylacetic acid
%K Methyl parathion
%K Degra dation
%K Tri-parent conjugation
%K Genetic engineering microogranism<br>耐盐，苯乙酸，甲基对硫磷，降解，三亲杂交，工程菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1F1C814CCD99A0F1&yid=D43C4A19B2EE3C0A&vid=BE33CC7147FEFCA4&iid=94C357A881DFC066&sid=D8414BC1307BF1A3&eid=8966A0F1B07BE5EE&journal_id=0001-6209&journal_name=微生物学报&referenced_num=10&reference_num=11