%0 Journal Article
%T Screening Strains Producing Inulinase and Cloning of Inulinase Gene<br>菊粉酶酶源菌株筛选及其基因克隆
%A Zhang Linghua Wang Yunji Ye Shuhong Zhang Fuqi
%A <br>张苓花
%A 王运吉
%A 叶淑红
%A 张福琪
%J 微生物学报
%D 2002
%I 
%X A wild-type strain AF10 producing inulinase was screened from soil samples, the strain is identified to be a A.niger. A endoinulinase gene inuA1 from A.niger AF10 was wequenced and analyzed, after PCR amplification. The results shows inuA1 is 1551 bp in length without any intron and contains 4 potential N-glycosylation sites. The conservative sequence is WMNEPN from the N-terminus. The inuA1 was cloned to pUC118 and the recombinant vector pinuA1 was obtained and transformed into E.coli JM109. The recombinant JM109/inuA1 included inuA1 was obtained.
%K Aspergillus niger
%K Inulinase
%K Gene cloning<br>黑曲霉，
%K 菊粉酶，
%K 基因克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=68BAA0564F658F75&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=4AD4BA66429F5627&eid=86C0C9A759FDA8CA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=15