%0 Journal Article
%T Analysis of 16S rDNA Sequences and DNA-DNA Hybridization of Moderately Halophilic Bacteria from Xinjiang Region<br>新疆地区盐湖的中度嗜盐菌16S rDNA全序列及DNA同源性分析
%A Jing Zeng
%A Yuetan Dou
%A Lei Wang
%A Susheng Yang
%A <br>曾静
%A 窦岳坦
%A 王磊
%A 杨苏声
%J 微生物学报
%D 2002
%I 
%X Based on the previous studies on numerical taxonomy and 16S rDNA PCR-RFLP analysis, the moderately halophilic bacteria isolated from Xinjiang Region constitued a new cluster, and the phylogenetic tree was constructed by comparing with the 16S rDNA sequences of the other moderately halophilic bacteria species. In the phylogenetic tree, most of the reference strains were clustered in a group, and the similarity values of 16S rDNA sequence were above 96%. However, AI-3, Alcanivorax borkumensis and Halobacillus litoralis were clusterd in another group, and the similarity value of 16S rDNA sequences between AI-3 and Alcanivorax borkumensis was 96%, and that of 16S rDNA sequences between AI-3 and Halobacillus litoralis was 99%. The results indicated that AI-3 was different from the reference strains in phylogeny. The values of DNA homology in the new cluster were more than 70%, but the value between AI-3 and Halomonas elongata was less than 50%. Thus, the strain AI-3 possibly represent a new moderately halophilic bacteria species.
%K Xinjiang Autonomy Region
%K 16S rDNA sequence
%K DNA-DNA hybridization
%K Phylogenesis<br>新疆地区
%K 16S
%K rDNA全序列
%K DNA－DNA杂交
%K 系统发育
%K 盐湖
%K 嗜盐菌
%K DNA同源性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4545E5A3B80E7B54&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=0B39A22176CE99FB&sid=76B5E24D6EC46B4B&eid=205BE674D84A456D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=18&reference_num=6