%0 Journal Article
%T Multi-repeat stress acclimation and butanol high-yielding strain screening by multi-factor complex medium<br>多轮次胁迫驯化及多因子复合筛选丁醇高产菌
%A LIU Xiao-Bo
%A LUO Wei
%A GU Qiu-Ya
%A YU Xiao-Bin
%A <br>刘小波
%A 罗玮
%A 顾秋亚
%A 余晓斌
%J 微生物学通报
%D 2012
%I 
%X Objective] The purpose of this study was to isolate a butanol-producing strain from the soil of corn field in Shiquan County, Shaanxi Province, China, and to improve its butanol tolerance and butanol yield. Methods] Using the multi-factor complex screening and the treatment of butanol stress acclimation, the strain with a high yield and butanol tolerance was obtained. Results] The results showed that through several batches of acclimation and screening, the mutant T64 was derived from isolated wild-type strain D64, its butanol tolerance was significantly improved and could grow well in the complex screening medium containing 20 g butanol/L. It produced 21.8 g/L total solvent (acetone, butanol, ethanol) and a butanol yield reached 15.18 g/L using 7% corn mash as fermentation medium, which was higher than that of the wild-type strain D64 (13.35 g/L). Conclusion] In conclusion, the designed multi-factor complex screening is more effective than the mono-factor screening for obtaining high-producing butanol strain. In addition, the stress acclimation of increasing butanol concentration in long term provides new ideas to research the tolerance of butanol.
%K Butanol high-yielding strain
%K Stress acclimation
%K Multi-factor complex screening
%K Butanol tolerance<br>丁醇高产菌
%K 胁迫驯化
%K 多因子复合筛选
%K 丁醇耐受性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=324F8504B2709856EE65668122AB8879&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=708DD6B15D2464E8&sid=99C22CF1E519BF36&eid=D9BD5E184C6E559D&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0