%0 Journal Article
%T Analysis on Integration Sites of Transposon-insertion Mutant by Transposon Rescue<br>转座子挽救法对转座子突变菌株中插入位点的定位分析
%A Bernhard Eikmann
%A <br>阮红
%A Bernhard
%A Eikmanns
%J 微生物学报
%D 2002
%I 
%X In order to define the chromosomal locus of the transposon integration in a mutant of Corynebacterium glutamicum, transposon rescue experiments were performed. By isolating parts of the transposon together with adjacent parts of the bacterial chromosome and subsequent sequencing of the adjacent parts,the mutant was found to have three transposon integrations, one just in front of the citrate synthase gene gltA, the other two in hitherto unknown open reading frames designated orfA and orfB. The transposon rescue experiments are proven to be a easy, practical and ideal way in analyzing the transposon integration sites.
%K Transposon rescue
%K Insertion mutagenesis
%K Corynebacterium glutamicum<br>转座子挽救法，
%K 插入突变，
%K 谷氨酸棒杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=54282AFD9065592C&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=377D325742940769&eid=6C069F4B248105A5&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=14