%0 Journal Article
%T Isolation and identification of a β-glucosidase-producing strain and enzymatic characteristics of the β-glucosidase<br>一株β-葡萄糖苷酶产生菌株的分离鉴定及酶学性质研究
%A ZHENG Fang
%A CAO Xiao-Fang
%A ZHANG Ya-Ling
%A BAI Fang
%A BAI Gang
%A <br>郑芳
%A 曹小芳
%A 张亚玲
%A 白芳
%A 白钢
%J 微生物学通报
%D 2012
%I 
%X Objective] To isolate a novel β-glucosidase-producing strain from soil,to clarify the taxonomic status,and to study the enzymatic characteristics of the β-glucosidase.Me-thods] A β-glucosidase-producing strain was isolated from soil sample using esculin and 4-nitrophenyl-β-D-glucopyranoside(PNPG) coloration methods;the taxonomic status of strain ZF-6C was clarified by morphological,physiological,chemotaxonomic characteristics and 16S rDNA analysis;the β-glucosidase was isolated and purified by ultra-filtration,hydrophobic interaction chromatography,anion chromatography,molecular sieve chromatography;with PNPG as the substrate,the optimal reaction pH and temperature of β-glucosidase were determined,the Michaelis constant Km of β-glucosidase against different substrates were determined by Lineweaver-Burk plot.Results] A strain,which produced β-glucosidase at high level,was isolated from soil and identified as Bacillus korlensis,the molecular weight of β-glucosidase isolated from Bacillus ZF-6C was 90 kD,the optimum reaction conditions for this enzyme were pH 7.0 and 40 °C.The enzyme was active against a wide range of β(1,4) linked disaccharides,the optimal substrate was o-nitrophenyl-β-D-glucopyranoside,the Km value was 0.73 mmol/L.Metal ions Ca2+ and Pb2+ enhanced enzyme activity,Cu2+ and Fe2+ inhibit the enzyme activity.Conclusion] This is the first report of isolation and identification of β-glucosidase from Bacillus korlensis.The β-glucosidase from Bacillus korlensis is widely different to known enzymes at molecular weight,optimum reaction conditions and substrate specificity aspects.This β-glucosidase may have novel structure and high catalytic efficiency.
%K Bacillus korlensis
%K Isolation and identification
%K β-glucosidase
%K Enzymatic properties<br>芽胞杆菌
%K 分离鉴定
%K β-葡萄糖苷酶
%K 酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=B5A93E035C82D3EFC6C9FD2EE4DC688A&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=5D311CA918CA9A03&sid=A326383D2F3B3AB0&eid=305A58D956DD6BAF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0