%0 Journal Article
%T CONSTRUCTION OF GENOMIC LIBRARY FROM L.lactis AL2 AND ISOTATION OF ENTIRE NISIN BIOSYNTHESIS GENE CLUSTER<br>乳酸乳球菌AL2基因文库的构建及乳链菌肽生物合成基因簇的筛选
%A X Chen
%A H Hu
%A S Jia
%A M Chen
%A L Huan
%A <br>陈秀珠
%A 胡海菁
%A 贾士芳
%A 陈美玲
%A 还连栋
%J 微生物学报
%D 2000
%I 
%X A total DNA library of Lactococcus lactis AL2 with high yield of nisin was successfully constructed using lambda EMBL3 as vector. 3900 plaques were obtained, which is much more than the required number of recombinants that represents an entire L. lactis genome according to Clarke and Carbon formula. Southern hybridization, PCR amplification and DNA sequencing revealed that the entire nisin biosynthesis gene cluster was isolated from the constructed library.
%K L
%K lactis\%
%K Genomic library
%K Nisin
%K Biosynthesis gene cluster<br>乳酸乳球菌，
%K 基因文库，
%K 乳链菌肽，
%K 生物合成基因簇
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EC9C96CB1B7A2241&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=94C357A881DFC066&sid=BC88D6B0750E09D1&eid=8F2250DA83AF77B8&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=7