%0 Journal Article
%T Comparative analysis of proteomic profile at different development stages of Volvariella volvacea by iTRAQ-coupled 2D LC-MSMS<br>iTRAQ结合2D LC-MS/MS技术在草菇不同生长发育时期蛋白质组分析中的应用
%A LIU Jing-Yu
%A JIANG Yu-Ji
%A XIE Bao-Gui
%A CHEN Bing-Zhi
%A LIAO Wei
%A DENG You-Jin
%A <br>刘靖宇
%A 江玉姬
%A 谢宝贵
%A 陈炳智
%A 廖伟
%A 邓优锦
%J 微生物学通报
%D 2012
%I 
%X Objective] This work aims to investigate differential expression proteins at different development stages of Volvariella volvacea using isobaric tags for relative and absolute quantification (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) proteomics approach. Methods] Firstly, the proteins extracted from V. volvacea at different development stages were analyzed by SDS-PAGE. Secondly, the tandem mass spcetrometry data obtained from 2D LC-MS/MS were used to search using MASCOT search engine. After that, principal component analysis, hierarchical clustering and Gene Ontology were used to analyze the detected results. Results] Results showed that 1 039 protein groups, included a total of 2 335 unique peptides, were identified. Among them, 1 030 protein groups were provided quantitative information. Contrast to mycelia, 64 up-regulated and 150 down-regulated significantly differential proteins were found in fruiting bodies at different development stages of V. volvacea. Bioinformatics analysis revealed that iTRAQ-coupled 2D LC-MS/MS was a unique method for isolating and identifying protein groups of V. volvacea at different development stages. Conclusion] It is helpful to insight into the molecular mechanism of the fruiting body formation and development of V. volvacea and other macro-basidiomycetes in the future.
%K Straw mushroom
%K Proteome
%K iTRAQ
%K 2D LC-MS/MS<br>草菇
%K 蛋白质组
%K iTRAQ
%K 二维液相色谱串联质谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=829D0ABBF0B6FE5AFAEAC986224FAFB3&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=B31275AF3241DB2D&sid=7BD5EBA75E30534B&eid=A2F69F17F19B4C46&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=32