%0 Journal Article
%T CLONING AND EXPRESSION OF otsA GENE IN E. COLI<br>大肠杆菌\%otsA\%基因的克隆和表达
%A Y Wang
%A X Dai
%A Y Wang
%A J Zhou
%A <br>王忆琴
%A 戴秀玉
%A 王韫恂
%A 周坚
%J 微生物学报
%D 2000
%I 
%X 1.5 kb of otsA gene encoding trehalose synthase has been cloned by PCR amplification. The DNA fragment was ligated to multi-copy vector and transformed to otsBA deleted and otsA deficient strains of E. coli separately. The transformants exhibited growth as well as the otsBA+ wild type on medium containing 0.5 mol/L NaCl. Trehalose was synthesized and accumulated in the transformed cells under osmotic pressure, which was determined by thin layer chromatograph. The results confirmed that otsA gene was functionally expressed in the recipient strains. These studies suggested that engineering otsA gene and trehalose accumulation into crop plants may improve drought and salinity tolerance.
%K otsA gene
%K Trehalose
%K Expression<br>otsA基因
%K 海藻糖
%K PCR扩增
%K 表达
%K 大肠杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=5D7C46304BFEC190&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=94C357A881DFC066&sid=47F7649551A37CFC&eid=FE6B7E9BDCCDBAA6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=15