%0 Journal Article
%T SELECTION AND RECOMBINANT EXPRESSION OF AN IMMUNODOMINANT B CELL EPITOPE OF HIV GP41<br>HIV蛋白质优势B细胞抗原表位的筛选、重组表达与抗原性鉴定
%A Du Yong
%A Xu Jing
%A Li Jingyun
%A Hou Lihua
%A Wang Haitao
%A <br>杜勇
%A 徐静
%A 李敬云
%A 侯利华
%A 王海涛
%J 微生物学报
%D 2000
%I 
%X A novel method was designed for disease specific B cell epitope mapping and epitope expression in E. coli. A phage library displaying random dodecamers was biopanned first with human total IgG antibodies against HIV 1, and then non specific phages were subtracted by HIV(-)polyclonal antibodies. After three rounds of screening, the positive phages were tested in an ELISA for their reactivity with HIV( ) IgG and HIV(-) IgG antibodies. Phages that showed positive reactivity with HIV( ) IgG, but negative to HIV(-) IgG, were selected and their displayed peptides were determined by DNA sequencing. All the 13 positive clones sequenced displayed five kinds of peptides (SPKCLGKLLCAF, THQCLGKLQCGV, SCSAKFTCTTQI, KSDCSARFMCSV, DCLKQWACEWSR)that have homology to the HIV 1 gp41( 602 GCSGKLICTTNV 613 ), demonstrating there is a dominat epitope in the region.
%K HIV
%K Epitope mapping
%K Random peptide library
%K Recombinant expression
%K ELISA<br>HIV，
%K 抗原表位，
%K 随机肽库，
%K 重组表达，
%K ELISA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=BD3D9D37C4D07408&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=38B194292C032A66&sid=B7BFA4B351E4C682&eid=44A4891E33BFF455&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=7