%0 Journal Article
%T Cloning and Expression of Vitreoscilia Hemoglobin in Streptomyces aureofaciens<br>透明颤菌血红蛋白基因在金色链霉菌中的克隆与表达
%A Meng Chun
%A Ye Qin Shi Xian'ai Qiu Li Song Siyang Guo Yanghao
%A <br>孟春
%A 叶勤
%A 石贤爱
%A 邱荔
%A 宋思扬
%A 郭养浩
%J 微生物学报
%D 2002
%I 
%X Vitreoscilla hemoglobin Gene was cloned in Streptomyces aureofaciens through E. coli-Streptomyces shuttle plasmids constructed by both pIJ699-pUC19(vhb) and pIJ702-pBR322(vhb). Under low dissolved oxygen conditions, expression of hemoglobin enhanced CTC yield of engineering strain more about 40%-60% that that of control by improving oxygen transmission in cells. Hemoglobin expression by Promoter of tetracycline resistance gene was more effective for oxygen transmission than that by its native promoter regulated by dissolved oxygen concentrations in environments of locally low concentrations of dissolved oxygen.
%K Vitreoscilla hemoglobin Gene
%K Streptomyces aureofaciens
%K Promoter of tetracycline resistance gene
%K Native promoter<br>透明颤菌血红蛋白基因，
%K 金色链霉菌，
%K 四环素抗性基因启动子，
%K 天然启动子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A615965462468E53&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=407C905D8F0449C4&eid=85002451B65CE0D1&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=12