%0 Journal Article
%T Improvement of Pythium guiyangense by constructive expression of Pr1 gene<br>根癌农杆菌介导的Pr1基因在贵阳腐霉的组成性表达
%A LIU Ping
%A ZHENG Hui-Ling
%A ZHAO Jing-Nan
%A WU Jian-Wei
%A SU Xiao-Qing
%A <br>刘萍
%A 郑慧玲
%A 赵竟男
%A 吴建伟
%A 苏晓庆
%J 微生物学通报
%D 2012
%I 
%X Objective] To construct a constructive expression vector pCambia-hph-Pr1 containing a Pr1 ORF of Pythium guiyangense for transformation of the fungus to get a genetic recombinant strain with enhanced virulence against mosquito larvae. Methods] The Pr1 gene expression vector was constructed using a binary vector as basic frame, and Pr1 gene was placed between promoter PgpdA and terminator TtrpC. The recombinant strains were obtained by Agrobacterium-mediated transformation system, with P. guiyangense mycelia as acceptor, using vector pCambia-hph-Pr1 containing hygromycin B resistance gene as marker. The biological characteristics and virulence to Culex larvae of the transformant were observed. Results] No distinct difference was found between wild-type stain and transformant in terms of growth rate and zoospore yield. A transformed strain of the fungus with significantly improved virulence was achieved. It caused an average larval death rate of 32.9% which was approximately double that of wild-type stain. Besides, the larvae exposed to transformant grew obviously slower. Conclusion] A genetically stable strain with higher virulence was obtained by constitutive expression of Pr1 protease through Agrobacterium-mediated transformation.
%K biocontrol
%K Constructive expression
%K virulence against Culex larvae<br>生物防治
%K 组成性表达
%K 灭蚊毒力
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=829D0ABBF0B6FE5A262FE338E0136D8B&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=B31275AF3241DB2D&sid=D46C5B75FD59EDCB&eid=741B1572DD1E3190&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15