%0 Journal Article
%T THE CONSTRUCTION OF A RECOMBINED E. COLI STRAIN WITH STABLE AND HIGH PRODUCTION OF POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)<br>高产稳产聚羟基烷酸的重组大肠杆菌的构建
%A Tian Jiesheng
%A Song Haichen
%A Wu Baihe
%A Wang Zhenfang
%A Li Jilun
%A <br>田杰生
%A 宋海琛
%A 吴柏和
%A 王珍芳
%A 李季伦
%J 微生物学报
%D 2000
%I 
%X Plasmid pJMC2 was constructed by cloning the parDE fragment of RK2 into pTZ18U-PHB which harbored phaCAB from Alcaligenes eutrophus and was transferred into E. coli HMS174 and E. coli JM107 separately. It is very stable in its hosts cultured in medium without ampicillin. E. coli HMS 174(pTZ18U-PHB) and E. coli JM107(pTZ18U-PHB) produced P(3HB-co-3HB) in a low phosphate concentration medium(18 mmol/L). The proportion of 3-hydroxyvalerate(3HV) in the polymer was 5%-8%. A fed-batch culture of E. coli HMS174(pJMC2) was conduct in a 5 L automatically controlled fermentor, the final dry cell weight, P(3HB-co-3HV) content, and th 3HV proportion were 42.5 g/L, 70% and 4.9% respectively.
%K Polyhydroxyalkanoate
%K Recombined E
%K coli
%K Stability of plasmid<br>聚羟基烷酸
%K 重组大肠杆菌
%K 质粒稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=14F8EAD7571C23E4&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=CA4FD0336C81A37A&sid=96C778EE049EE47D&eid=4AD960B5AD2D111A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=12