%0 Journal Article
%T Invasion of Aeromonas hydrophila in Cells Involving Signal Transduction and Cytoskeleton<br>嗜水气单胞菌侵袭力与宿主细胞信号转导和骨架的关系
%A Chu Weihua Lu Chengping
%A <br>储卫华
%A 陆承平
%J 微生物学报
%D 2003
%I 
%X Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm') bacteria from Triton X-100 cell lysates. The invasive efficiency (IE) could reach to 0.2% at 37 degrees C. In addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. Bacterial internalization of Ah J-1 involved microfilaments and protein tyrosine kinase since cytochalasin D (an inhibitor of microfilament polymerization) and genistein (an inhibitor of protein tyrosine kinase) prevented internalization. Staurosporine (a protein kinase C inhibitor) and Sodium orthovanadate (a protein tyrosine phosphatase inhibitor) accelerated internalization of Ah J-1 into HEp-2 cells.
%K Aeromonas hydrophila
%K Invasion
%K Signal transduction
%K Cytoskeleton<br>嗜水气单胞菌
%K 侵袭，信号转导，细胞骨架
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=0FD7C108DB54B20E&yid=D43C4A19B2EE3C0A&vid=BE33CC7147FEFCA4&iid=B31275AF3241DB2D&sid=92DE343A8428AA81&eid=45D68DF69BA881EC&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=13