%0 Journal Article
%T Isolation and Identification of a Theophylline-degrading Bacterial Strain<br>一株茶碱降解菌的分离和鉴定
%A Xu Meiying
%A Cen Yinghua
%A Deng Shuier
%A Sun Guoping
%A Wuhan Institute of Virology
%A Chinese Academy of Sciences
%A Wuhan
%A China
%A <br>许玫英
%A 岑英华
%A 邓穗儿
%A 孙国平
%J 微生物学报
%D 2002
%I 
%X Bacterial strain Tcn3 was isolated from the activated sludge of a pharmaceutical wastewater treatment system, which could grow with theophylline as the sole source of carbon and energy. The maximum concentration of theophylline degraded by Tcn3 is 3 000mg/L. When the concentration of theoplyline is 1 000mg/L, a complete degradation only needs 48h. For Tcn3 degrading theophylline, the optimal pH is 8^0 and K++ is the necessary element. The sequence analysis of a 1 500bp 16SrDNA fragment amplified from total DNA of strain Tcn3 and the traditional identification methods was used to identify Tcn3. The results showed that Tcn3 shares 99.7% 16S rDNA sequence homology with Paracoccus versutus type strain ATCC 25364. In phylogenetic framework of bacterial classification, Tcn3 belongs to proteobacteria; alpha subdivision; Rhodobacter group: Paracoccus versutus.
%K Theophylline
%K Biodegradation
%K 16S rDNA
%K Paracoccus versutus<br>茶碱，
%K 生物降解，
%K 16S
%K rDNA，
%K 善变副球菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=63A8C51D63781217&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=94C357A881DFC066&sid=E3D3D8D1B650AE1E&eid=FF58680609C9D068&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=10