%0 Journal Article
%T PREPARATION, PURIFICATION AND IDENTIFICATION OF SIALIC ACID FROM ESCHERICHIA COLI C 8 *<br>从大肠杆菌C-8制备和纯化唾液酸
%A Qian Shijun
%A Li Jian
%A Gao Jianmei
%A Guo Liangdong
%A Ye Jun
%A <br>钱世钧
%A 李剑
%A 高建梅
%A 郭良栋
%A 叶军
%J 微生物学报
%D 1999
%I 
%X Colominic acid produced from Escherichia coli C-8 was purified by ammonia sulfate precipitation, dialysis and concentration. After hydrolysis of colominic acid at a pH of 2.5, at 70 degrees C for 4 h, sialic acid was obtained, then purified by chromatography on Dowex1-x8. Analysis of thin-layer chromatography and absorption spectrum of sialic acid in the orcinol/Fe3+/HCl and the periodic acid/thiobarbituric acid confirmed that its purity was identical with that of standard sample.
%K Escherichia coli
%K Sialic acid
%K Purification<br>大肠杆菌，唾液酸，纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=89FA861EBABDADFA8A2576E40464705B&yid=B914830F5B1D1078&vid=7C3A4C1EE6A45749&iid=0B39A22176CE99FB&sid=4609832E4B5C797B&eid=F1A8654ADB4E656E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=3