%0 Journal Article
%T COMPOSITION AND STRUCTURE OF BIOFLOCCULANT BP25<br>絮凝剂BP25的化学组成及结构研究
%A Z Liu
%A G Xu
%A Z Liu
%A H Yang
%A <br>刘紫鹃
%A 徐桂云
%A 刘志培
%A 杨惠芳
%J 微生物学报
%D 2001
%I 
%X A strain of Bacillus megaterium screened from activated sludge could produce exocellular flocculant. The flocculant was purified through ethanol precipitation and Sephadex S-500 column chromatography. The purified flocculant BP25 was assayed by Bradford reaction, agarose gel electrophoresis and Sulfate-phenol method. The results showed that BP25 was a kind of polysaccharide which contains 36.97% O, 6.28% H, 47.00% C, 0% No NMR assay showed BP-25 contains no uronic acid. Gas-chromatography assay combined with thin-layer chromatography of acid hydralate revealed that BP25 contains Glucose and Mannose with the mole ratio of 4:1. Methylation analysis revealed the polysaccharide contains alpha-1, 6 glycosidicbond and alpha-1, 3 glycosidicbond. The main chains are comprised of Glucose and all the Mannose are in the side chains. Possible repeating unit structure was deduced.
%K Flocculant
%K Polysaccharide
%K Methylation analysis
%K GC/MS<br>絮凝剂
%K 多糖
%K 甲基化分析
%K 色谱质谱联用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AA8BA6852E13209C&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=375BEEEA164CFE59&eid=EA64BD0B2FF6E786&journal_id=0001-6209&journal_name=微生物学报&referenced_num=11&reference_num=8