%0 Journal Article
%T IDENTIFICATION AND LOCATION OF THE TOXIN PROTEIN GENES IN 56 BACILLUS THURINGIENSIS ISOLATES<br>Bt毒素蛋白基因的PCR鉴定及定位
%A Zhong Xiaofen
%A Yuan Meijin
%A Zhang Ping
%A Deng Riqiang
%A Long Qingxin
%A Pang Yi
%A Wang Xunzhang
%A <br>钟肖芬
%A 袁美妗
%A 张萍
%A 邓日强
%A 龙綮新
%A 庞义
%A 王珣章
%J 微生物学报
%D 2001
%I 
%X Polymerase chain reaction was performed to screen \?cillus thuringiensis\% isolates for \%cry\%I,\%cry\%III,and \%cry\%V genes with \%cry\%I\|,\%cry\%III\|,and \%cry\%V\| specific primers respectively.The results indicated that,in the 56 Bt isolates examined,7 isolates contain \%cry\%I gene,2 isoltaes contain \%cry\%III gene,and 21 isolates contain both \%cry\%I and \%cry\%V genes.The \%cry\%V gene was located by Southern hybridization in the large plasmid of about 150MD molecular mass.
%K Bacillus thuringiensis
%K \% Toxin protein genes
%K Identification<br>Bt
%K 毒素基因
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9976C9CFD75F35E1&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=2AC7DCCBBC26ECF8&eid=E42CAFB11D4BE21A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=7&reference_num=7