%0 Journal Article
%T EXPRESSION OF HUMAN LEUKEMIA INHIBITORY FACTOR GENE IN PICHIA PASTORIS<br>人白血病抑制因子基因在巴斯德毕赤酵母中的表达
%A Wang Quanzhong
%A Yang Lin
%A Quyang Jing
%A Long Qingxin
%A Wang Xunzhang
%A <br>王全忠
%A 杨林
%A 欧阳菁
%A 龙綮新
%A 王珣章
%J 微生物学报
%D 2001
%I 
%X The 575 bp artificial synthesized human leukemia inhibitory factor (hLIF) gene was cloned into vector pPICZ alpha A and the constructed expression vector pPICZ alpha A-hLIF was linearized by SacI and transformed into Pichia pastoris X-33. After the screening for Mut phenotype and the PCR analysis of the transformants, hLIF gene expressed in Pichia pastoris. SDS-PAGE and Western blot analysis of the culture supernatants showed that hLIF gene could express as 58.5 kD protein with the desired immunogenicity. Density scanning of the SDS-PAGE gel revealed that the targeted protein accounted for 32.8% of the total protein in the supernatants. The expressed products can inhibit the clone formation of murine's teratoma cells.
%K Human leukemia inhibitory factor
%K Pichica pastoris
%K Gene expression
%K Bioactivity
%K assay<br>人白血病抑制因子
%K 巴斯德毕赤酵母
%K 基因表达
%K 生物活性测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=85909CCAC8D0CD99&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=90773C2285A2F0BB&eid=CD26609C367AC9C8&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13