%0 Journal Article
%T EXPRESSION OF RECOMBINANT HUMAN STEM CELL FACTOR IN INSECT CELLS<br>重组人干细胞因子在昆虫细胞中的高效表达
%A Zhang Dongmei
%A Qin Junchuan
%A Zang Yuhui
%A Zhu Jie
%A Shen Beifen
%A <br>张冬梅
%A 秦浚川
%A 臧宇辉
%A 朱洁
%A 沈倍奋
%J 微生物学报
%D 2000
%I 
%X The cDNA of human stem cell factor(hSCF) containing signal sequence was cloned into the transfer vector pVL941 of AcNPV to construct a recombinant transfer vector pVL941-SCF. Sf9 cells were cotransfected with wild type viral DNA and pVL914-SCF to produce the recombinant virus AcNPV-SCF by homologous recombination in cell. Southern-hybridization analysis suggested that the recombinant viral DNA contained hSCF cDNA fragment. The Sf9 cells infected with the recombinant baculovirus AcNPV-SCF expressed biologically active rhSCF which was secreted into the cell culture. The synergistic activities of SCF in conjunction with human interleukin-3(hIL-3) was measured by MTT colorimetric method and TF-1 cell line. The expression level of Sf9 cells reached its highest at about 1970 units/ml in the 3rd day after the infection with AcNPV-SCF. Three SCF bands with molecular masses of 18 x 10(3), 20 x 10(3) and 22 x 10(3) were detected by immunoblotting.
%K Human stem cell factor
%K AeNPV
%K Expression in insect cell<br>人干细胞因子
%K AcNPV
%K 昆虫细胞表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=91C30F7B3E039D58&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=CA4FD0336C81A37A&sid=1AE5323881A5ECDC&eid=2A3781E88AB1776F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=11