%0 Journal Article
%T PRDODUCING CHARACIERIZATION OF CONSTTUTIVE-FORM LACCASE BY BASIDIOMYCETE<br>担子菌组成型漆酶产生特性的研究
%A Chao Yapeng
%A Ye Jun
%A Qian Shijun
%A <br>钞亚鹏
%A 叶军
%A 钱世钧
%J 微生物学报
%D 2000
%I 
%X A constitutive-laccase-producing fungus was gained from 20 strains of different sources with plate method. Laccase activity reached 0.58 IU/mL when the strain grew on DC medium with shaking cultivation, which was superior to stationary method by 4 times. The enzyme production paralleled with biomass in early 8 days, subsequently activities declined slightly though its biomass continued upward. Tyrosine and tryptone at the concentration of 4 g/L improved the enzyme production, reaching 2.49 and 2.0 IU/mL respectively. Some tested chemical agents seemed not to contribute to the increase of the enzyme production effectively except for syringaldazine and o-tolidine. Heatshock at 40 degrees C for 1 h made 1.3 times more enzyme expression.
%K Basidiomycete
%K Laccase
%K Constitutive<br>担子菌，
%K 漆酶，
%K 组成型
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=5A2D0EC416943B52&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=B31275AF3241DB2D&sid=3EABEBD973E45554&eid=7E2D9DFE40003B3F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=19&reference_num=17