%0 Journal Article
%T CLONING AND EXPRESSION OF THE ENVELOPE GLYCOPROTEIN gD GENE OF PSEUDORABIES VIRUS EA STRAIN<br>伪狂犬病病毒鄂A株包膜糖蛋白gD基因的克隆与表达
%A X Chen
%A L Yang
%A W Hong
%A H Chen
%A Q Chen
%A Q Long
%A X Wang
%A <br>陈新华
%A 杨林
%A 洪文洲
%A 陈焕春
%A 陈曲侯
%A 龙綮新
%A 王珣章
%J 微生物学报
%D 2001
%I 
%X The envelope glycoprotein gD gene of pseudorabies virus Ea strain was cloned via PCR technique. Sequence analysis displayed 98% nucleotide sequence homology and 97% deduced amino acid sequence homology between our cloned gD gene and PRV Rice strain gD gene. The recombinant transfer plasmid pSX35A-gD was obtained by inserting D gene into the baculovirus transfer vector pSX35A with whole-phase promoter cassette, then transfected insect cell Hi5 with linearized AcMNPV-OCC- virus DNA, and formed recombinant baculoviruses AcMNPV-OCC(+)-gD by homologous recombination in insect cell. Recombinant baculoviruses infected insect cell Hi5 after being purified by plaque assay. Both SDS-PAGE and Western-blotting showed glycoprotein gD with a molecular weight of about 47 kD was expressed specifically, product was about 6.2% of total cellular protein, and expressed gD was of immunogenicity.
%K Pseudorabies virus
%K gD gene
%K Baculovirus expression system<br>伪狂犬病病毒
%K gD基因
%K 杆状病毒表达系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C789E2513D392F0B&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=C6EC7357BCACD3A4&eid=CF6CB42CFF3D4C4E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=8