%0 Journal Article
%T Sequence and Evolutional Analysis of Escherichia coli O54 O-antigen Gene Cluster<br>大肠杆菌O54 O-抗原基因簇的破译及进化分析
%A YANG Jing
%A Hua
%A YU Hao
%A REN Yi
%A WANG Lei
%A FENG Lu
%A <br>杨静华
%A 于浩
%A 任一
%A 王磊
%A 冯露
%J 微生物学报
%D 2004
%I 
%X The Escherichia coli O54 O antigen gene cluster was sequenced. It was found to contain genes responsible for the biosynthesis of nucleotide sugar dTDP rhamnose ( rmlBDA and rmlC ), genes encoding O unit flippase( wzx ) and O antigen polymerase( wzy ) and putative transferase. Unlike most of the cases in which rml genes are located together, rmlC was apart from other rml genes. Further analysis indicated that rmlC gene was recently introduced into the O antigen gene cluster of Escherichia coli O54 It was also found that E.coli O54 and Shigella bodyii 9 are closely related. By polymerase chain reaction for the 166 E.coli and 43 Shigella O serogroups, wzx and wzy as specific genes for E.coli O54 were identified This work provides the basis for a sensitive test for the rapid detection of E.coli O54
%K Escherichia coli
%K O54
%K O
%K antigen gene cluster
%K rmlC
%K gene
%K Evolutionary
%K Specific gene<br>大肠杆菌O54
%K O-抗原基因簇
%K rmlC基因
%K 进化分析
%K 特异基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6DD070D21DCC49AE&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=E158A972A605785F&sid=A48DE16C07AAAB06&eid=9B95A71E6639C039&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=20