%0 Journal Article
%T EXPRESSION OF RICE DWARF VIRUS OUTER COAT PROTEIN GENE(S8) IN INSECT CELLS<br>水稻矮缩病毒外壳蛋白基因S8在昆虫细胞中的表达
%A S Li
%A H Liu
%A Z Chen
%A Y Li
%A <br>李胜
%A 刘慧君
%A 陈章良
%A 李毅
%J 微生物学报
%D 2001
%I 
%X Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected.
%K Rice Dwarf Virus
%K Baculovirus
%K Expression in insect cells
%K Western
%K blot<br>RDV
%K 杆状病毒
%K 昆虫细胞表达
%K Western-blot
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=294A1C17F98B3AED&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=0B39A22176CE99FB&sid=F1177A9DF1349B63&eid=43608FD2E15CD61B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=13