%0 Journal Article
%T STUDIES ON IN VITRO EXPRESSION FOR gI GENE OF MAREK''''S DISEASE IN E. COLI BY pGEX VECTOR<br>pGEX载体表达马立克氏病病毒囊膜糖蛋白gI基因的最佳条件
%A J Ding
%A Z Cui
%A <br>丁家波
%A 崔治中
%J 微生物学报
%D 2001
%I 
%X Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus (MDV) 648 strain by polymerase chain reaction(PCR). PCR product was cloned into pGEX-6p-1 according to the right open reading frame(ORF). The expression of GST-gI fusion protein was studied in detail on many factors including temperature, timing and IPTG. The curve for OD600 and the growing time of the recombinant bacteria is also established., which is helpful to find the optimal inducing time. GST-gI fusion protein was identified by SDS-PAGE and Western-blotting., and the result shows that the best concentration of IPTG is 0.2-0.5 mmol/L and inducing time have great effects on expression while temperature has little. The fusion protein was injected into mouse five times to identify its antigenicity and the result is positive in indirect fluorescent assay IFA.
%K Marek's disease virus(MDV)
%K gI gene
%K Fusion protein
%K Indirect fluorescent assay (IFA)
%K Western\|blotting<br>马立克氏病病毒
%K gI基因
%K 融合蛋白
%K 间接免疫荧光试验
%K 蛋白质印迹试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D991DD47C1162D15&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=41A78CBB5BAB6860&eid=6A9657F54F754BF6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=13