%0 Journal Article %T ISOLATION AND CHARACTERIZATION OF MUTANTS OF ASPERGILLUS NIGER DEFICIENT IN EXTRACELLULAR PROTEASES
黑曲霉蛋白酶缺陷突变体的分离和鉴定 %A Hong Bin %A Zhang Yang %A Li Yuan %A
洪斌 %A 张洋 %A 李元 %J 微生物学报 %D 2000 %I %X Several mutants of Aspergillus niger 3 795 , deficient in extracellular protease expression, have been isolated and characterized biochemically after in vivro UV mutagenesis of conidiospores and selected by halo screening on the gelatin/casein plate and skim milk plate. Extracellular proteolytic activities of 3 795 1 23 and 3 795 1 30 are 5.4% and 8.4% of the parental strain and these strains may be used as the host for efficient expression of foreign proteins. %K Aspergillus niger %K Extracellular proteases %K Protease %K deficient mutants %K Heterologous protein degradation
黑曲霉, %K 胞外蛋白酶, %K 蛋白酶缺失菌株, %K 外源蛋白降解 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A4B96CC60BE60B6A&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=E158A972A605785F&sid=8ACD9060100C26F1&eid=EB58C3052341AAA3&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=9