%0 Journal Article
%T Development of a PCR method for the detection of pathogenic Aeromonas veronii<br>致病性维罗纳气单胞菌检测方法的建立
%A PAN Xiao-Yi
%A LIN Ling-Yun
%A YUAN Xue-Mei
%A YIN Wen-Lin
%A HAO Gui-Jie
%A XU Yang
%A YAO Jia-Yun
%A SHEN Jin-Yu
%A <br>潘晓艺
%A 蔺凌云
%A 袁雪梅
%A 尹文林
%A 郝贵杰
%A 徐洋
%A 姚嘉赟
%A 沈锦玉
%J 微生物学通报
%D 2011
%I 
%X The aim of the study was explore a set of new detective targets in pathogenic Aeromonas veronii which was more specific than others at present,to develop a new duplex PCR method which could effectively detection the pathogenic A.veronii.Comparison analysis of the 16S rRNA gene se-quences of Aeromonas spp.was used to explore A.veronii specific targets,which were then evaluated and used to design specific primers,and select the conservative primers of aerolysin genes in Aeromo-nas spp.to explore pathogenic targets.PCR parameters were optimized,its reaction system was devel-oped,and its sensitivity and specificity were checked.12 Aeromonas stains and 10 non-Aeromonas stains were tested for pathogenic A.veronii by duplex PCR method.The duplex PCR can clearly iden-tify A.veronii from Aeromonas species,and can identify aerolysin-producing stains of A.veronii.The detection limit of genomic DNA by the duplex PCR was 1.35×10-3 mg/L.A novel duplex PCR method was successfully developed in this study,which could effectively detect pathogenic A.veronii with high accuracy and sensitivity.
%K Aeromonas veronii
%K 16S rRNA
%K Aerolysin
%K Pathogenicity
%K Duplex PCR<br>维罗纳气单胞菌
%K 16S
%K rRNA
%K 气溶素
%K 致病性
%K 双重PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=8AC6258FD9B9B701306E4D4378333EA8&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=59906B3B2830C2C5&sid=D217FECA03012AA9&eid=73DFEE35F698150B&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=28