%0 Journal Article
%T Study on the Sequence Analysis,Expression and Application of Chitinase Gene From Bacillus circulans<br>环状芽孢杆菌几丁质酶基因序列分析、表达和生物活性测定
%A Wang Yanlin Wang Haiyan Qin Min Zhang Yizheng
%A <br>王焰玲
%A 王海燕
%A 秦敏
%A 张义正
%J 微生物学报
%D 2002
%I 
%X Sequence analysis of the chitinase gene cht1 of Bacillus circulans showed that it contained 2151 nucleotides,which codes the precursor of chitinase CHT1 with 717 amino acid residues.The nucleotide and deduced amino acid sequences of cht1 showed 81% and 95% homology with those of ChiA of B.circulans WL-12,respectively.The cht1 gene was cloned into the Escherichia coli-Bacillus subtilis shuttle vector pSUGV4 and two recombinant plasmids, named pUSCH1 and pUSCH2 which contained 2.9kb and 4.0 kb insert respectively,were obtained.The recombinant plasmids were transformed into B.subtilis DB104 and WB600.Chitinase activity was detected both in transformed E.coli and B.subtilis.The DB104/pUSCH1 strain was found to be effective in the bio-controlling the infection of Magnaporthe grisea under greenhouse condition,which showed 71.67% decrease in rice disease incidence.
%K Bacillus circulans
%K Chitinase gene
%K Bacillus subtilis
%K Magnaporthe grisea<br>环状芽孢杆菌，
%K 几丁质酶基因，
%K 枯草芽孢杆菌，
%K 稻瘟病菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2189ADC9F0F744E1&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=94C357A881DFC066&sid=984BD2F4D19B9D1C&eid=06D504E5261AB652&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=14