%0 Journal Article
%T STUDIES ON MONOCLONAL ANTIBODIES OF MAGNAPORTHE GRISEA AND ITS INTERFERING EFFECT ON APPRESSORIUM FORMATION<br>稻瘟病菌单克隆抗体的研制及其对稻瘟病菌附着胞形成的影响
%A Wu Jianxiang
%A Liu Fucheng
%A Li Debao
%A Chen Zhengxian
%A Lou Yichun
%A <br>吴建祥
%A 林福呈
%A 李德葆
%A 陈正贤
%A 娄沂春
%J 微生物学报
%D 2000
%I 
%X Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies (McAbs) against Magnaporthe grisea were produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c mice immunized with the mixture of conidia, germ tubes and appressoria of M. grisea using 50% PEG. The result of IFTC test showed that four of McAbs, named as 2B4, 4A1, 1D1, and 2H4 specifically bound to the cell wall surface of the fungus; Western blotting revealed that 2B4, 4A1, 1D1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrane and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.
%K Appressorium of magnaporthe grisea
%K Monoclonal antibody
%K IFA<br>稻瘟病菌附着胞，
%K 单克隆抗体，
%K 间接免疫荧光试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FC1F83E9B8EBEDD3&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=B31275AF3241DB2D&sid=50B6AC44200581A5&eid=9107B2E171152411&journal_id=0001-6209&journal_name=微生物学报&referenced_num=16&reference_num=11