%0 Journal Article
%T ATP SYNTHESIS BY FREE YEAST CATALYSIS COUPLING HOLLOW FIBER ULTRAFILTRATION SEPARATION<br>耦合中空纤维膜超滤分离游离细胞催化合成ATP
%A Duan Xuehui
%A Zhang Siliang
%A Ye Qin
%A <br>段学辉
%A 张嗣良
%A 叶勤
%J 微生物学报
%D 2000
%I 
%X A novel process for the ATP production employing free yeast catalysis and coupling a hollow fiber module ultrafiltration separation was studied. The experimental results of biocatalyst half-life and operating stability for ultrafiltration showed that the enzymes released from free yeast cell could be cut-off efficiently from the product output stream by hollow fiber membrane. The intercepted percentage of ADH and HK reached more than 95% during the steady running. The efficiency of enzymes utilized was 2.0-2.5 fold as much as that of batch reaction. And the time of yeast cells used repeatedly for ATP synthesis reached 2.5-3.0 batches. Under 0.1 MPa, the ultrafiltrating speed of hollow fiber membrane could keep on a stabilizing value for 11 batches continuously. The continuous operation mode of ATP synthesis simultaneous product ultrafiltration separation could be maintained in higher conversion rate for 5.0 h when the whole system fell in a pseudo-stat where the dilution rate was equal to 0.25 h-1.
%K Simultaneous reaction and separation
%K Free yeast cell
%K ATP
%K Hollow fiber membrane
%K Biocatalysis<br>反应与分离耦合，
%K 游离酵母，
%K ATP，
%K 中空纤维膜，
%K 生物催化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=793B82CA97784AF1&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=B31275AF3241DB2D&sid=06F643376BC2509E&eid=826ED638BDB6F0D0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=8