%0 Journal Article
%T BIOSYNTHESIS OF GLUTATHIONE:CONSTRUCTION OF ATP REGENERATION SYSTEM BETWEEN RECOMBINANT E. COLI AND S. CEREVISIAE<br>生物合成谷胱甘肽种间耦合ATP再生系统的构建
%A Y Li
%A H Li
%A J Lin
%A J Chen
%A <br>李寅
%A 李华钟
%A 林金萍
%A 陈坚
%J 微生物学报
%D 2001
%I 
%X An ATP regeneration system between two microorganisms, in which recombinant E. coli II-1 and S. cerevisiae WSH-J702 were used as the biosynthesis enzymes source of glutathione and ATP, respectively, was constructed by using glucose as an energy substrate. Yeast cells after permeable treatment could hardly consume glucose. The glycolysis pathway of yeast was started by adding 1.0 mmol/L AMP and 0.05 mmol/L NADH simultaneously. GSH biosynthesis in the coupling system was improved with increasing glucose concentration to 400 mmol/L, where the concentration of GSH reached 10.4 mmol/L (3.2 g/L). Both the coupling system and the non-coupling system with ATP added could hardly produce GSH under the absence of magnesium. The low efficiency of ATP regeneration system constructed was probably caused by the chelation between magnesium and ATP. By adding magnesium to the coupling system, a final concentration of GSH, 14.3 mmol/L (4.4 g/L), was achieved at 6 h of reaction.
%K Recombinant Escherichia coli
%K Saccharomyces cerevisiae
%K ATP regeneration system between two microorganisms
%K Glutathione
%K Biosynthesis
%K Construction<br>重组大肠杆菌
%K 面包酵母
%K 种间耦合ATP再生系统
%K 谷胱甘肽
%K 生物合成
%K 构建
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=7C267538D58E0332&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=0B39A22176CE99FB&sid=AC1578C6BB9EBDEF&eid=2BA123C6EB9D54C2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=14