%0 Journal Article
%T STUDIES ON TRANSFERENCE OF HYDROGENASE GENES OF RHIZOBIUM ARACHIS<br>根瘤菌吸氢酶基因转移的研究
%A Z Wang
%A M Long
%A Y Liu
%A F Zhang
%A L Xu
%A T Zhu
%A S Jiang
%A <br>王振宇
%A 龙敏南
%A 刘月英
%A 张风章
%A 许良树
%A 朱天赐
%A 蒋水柳
%J 微生物学报
%D 2001
%I 
%X The hydrogen-uptake genes were transferred into wild Rhizobium arachis Ra strains (Hup-, Nif+, Apr) by triparental mating using pRK2013 as help plasmid. A transconjugant R. arachis Rz34-2(Hup+, Nif+, Apr, Tcr) which expressed high activities of hydrogenase and nitrogenase under free-living and symbiotic state was screened. Peanut inoculation test with recipient R. arachis Ra34, transcojugant Rz34-2 and control strain R. arachis L8-3 (Hup+, Nif+) was carried out respectively. The results showed that, compare to treatment without inoculation, inoculation with R. arachis Ra34 and R. arachis L8-3, the dry weight of leaf inoculated with transconjuant Rz34-2 increased 6.2%, 7.6% and 6.3% respectively; the N-content of seed increased 8.8%, 10.0% and 6.0%; the output increased 18.8%, 10.5% and 10.7%. This suggested that legume plants inoculated with Rhizobium strains (Hup+) were more efficient to accumulate N and to increase its output.
%K Rhizobium arachis
%K Hup gene
%K Plant test<br>花生根瘤菌
%K 转吸氢基因
%K 大田实验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F487C3DDF54C40AB&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=E158A972A605785F&sid=65C08888CCE4801E&eid=BA48F0B914ED890A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=15