%0 Journal Article
%T CLONING OF PROMELITTIN cDNA AND ITS EXPRESSION IN ESCHERICHIA COLI<br>蜂毒溶血肽前体蛋白cDNA的克隆及其融合蛋白的表达
%A Wang Guanlin
%A Li Dali
%A Fang Hongjun
%A <br>王关林
%A 李大力
%A 方宏筠
%J 微生物学报
%D 2001
%I 
%X The cDNA encoding promelittin was obtained from the total RNA of bee poison gland by RT-PCR and was cloned to pT7Blu-T vector. The expression vector of promelittin fused with partial sequence of beta-galactosidase was constructed by ligating the fragment inserted to pUC118. Moreover, it was expressed in the strain DH5 alpha of Escherichia coli. The result of DNA sequence analysis demonstrated that the obtained cDNA sequence was same with the published one and the reading frame of fusion gene was correct.
%K Promelittin
%K cDNA
%K Expression fused<br>蜂毒溶血肽前体蛋白
%K cDNA
%K 融合蛋白表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=41B6DE6B74E7EF08&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=0B39A22176CE99FB&sid=7EBE588F611589FC&eid=D46BA3D3D4B3C585&journal_id=0001-6209&journal_name=微生物学报&referenced_num=8&reference_num=10