%0 Journal Article
%T Expression of Recombinant Human Serun Albumin in Genetically Engineered Pichia pastoris in High-density Fermentation<br>重组巴氏毕赤酵母高密度发酵表达rHSA
%A Guo Meijin
%A Zhuang Yingping
%A Chu Ju
%A Zhang Siliang
%A Liu Zhimin
%A <br>郭美锦
%A 庄英萍
%A 储炬
%A 张嗣良
%A 刘志敏
%J 微生物学报
%D 2002
%I 
%X The optimum culture conditions of genetically engineered Pichia pastoris in shake-flask cultivation and in fed-batch fermentation were investigated respectively in this paper. It showed that the cultural period induced with 5 g/L methanol is 96 h, optimum methanol concentration is 10 g/L, and pH range is 5.72-6.59 in shake-flask culture. Although the seed inoculum amount increased, the target protein productivity per cell optical density was decreased. Their relationship fit the equeation Y = 12.941x(-0.5059) (r = 0.9789, where x is inoculating OD600, Y is protein productivity per cell optical density), we postulated that the restricting factor may be dissolved oxygen (DO) at shake-flask culture. With the 10% inoculum and 20 OD600 of seed, the lag phase of cell growth is 2.11 h in batch cultivation, and the relationship between cell optical density (Y) and culture time (t) is Y = 0.7841e0.2319t (r = 0.9936); The cell dry weight of broth reached 115-160 g/L and the maximum rHSA concentration was 3.6 g/L at the 120th at the fed-batch fermentation phase.
%K Genetically engineered Pichia pastoris
%K High-density fermentation
%K rHSA<br>重组巴斯德毕赤酵母
%K 高密度发酵
%K 重组人血清白蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90ba3d13e7f3bc869ac96fb3da594e3fe34fbf7b8bc0e591&jid=a3c6ba55ab623b90fa9104cffc826f3c&aid=9b3fb17ad3d74fa0&yid=c3acc247184a22c1&vid=ece8e54d6034f642&iid=ca4fd0336c81a37a&sid=95d537ac89b28832&eid=68d88c2fcf9c3098&journal_id=0001-6209&journal_name=微生物学报&referenced_num=10&reference_num=19