%0 Journal Article
%T PURIFICATION AND PROPERTIES OF DEGRADING ENZYME OF ORGA NOPHOSPHORUS PESTICIDES FROM ASPERGILLUS ORNATUS Z58<br>华丽曲霉Z58有机磷农药降解酶的纯化和性质
%A Liu Yuhuan
%A Zhong Yingchang
%A <br>刘玉焕
%A 钟英长
%J 微生物学报
%D 2000
%I 
%X Degrading enzyme of organophosophorus pesticides was purified from Aspergillus oryzeZ58 by (NH4)2SO4 precipitation, Sephadex G-100 gel filtration, DEAE-cellulose chromatography with 34.2-fold purification, 17.8% recovery and 76.2 U/mg specific activity. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. The molecular weight of the enzyme estimated with Sephadex G-100 was 67000. The optimal conditions for activity were pH 7.2, temperature 45 degrees C. The enzyme was stable over the range of pH 6-10 and below 40 degrees C, the activity was inhibited by SDS and stimulated by Cu2+, the substrate ranges were nonspecific to organophosophorus pesticides.
%K Degrading enzyme of organophosophorus pesticides
%K Aspergillus oryze
%K Purification and properties<br>有机磷农药降解酶，
%K 华丽曲霉，
%K 纯化和性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D6366BBAA99D32D3&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=E158A972A605785F&sid=389DA78D878702A9&eid=E57FE519484CFB70&journal_id=0001-6209&journal_name=微生物学报&referenced_num=27&reference_num=7