%0 Journal Article
%T STUDY ON THE PHYSICOCHEMICAL PROPERTIES OF CAMPYLOBACTER JEJUNI ENTEROTOXIN<br>空肠弯曲菌肠毒素理化特性的研究
%A Wu Run
%A Si Hongwei
%A <br>吴润
%A 司宏伟
%J 微生物学报
%D 2000
%I 
%X Precipitate of Campylobacter jejuni cytotonic enterotoxin(CE) performed in an 80% saturated solution of ammonium sulfateit indicated that there were some little molecular proteins except the 68 kD main band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), whereas the eluate from GM1 ganglioside affinity column chromatography exhibited only one 68 kD band on SDS-PAGE. The results suggest that CE mainly be consisted of 68 kD protein. The toxin is heat-labile, pH dependent and resistant to trypsin, It could be completely inactivated by heating at either 56 degrees C and 60 degrees C for 30 min or 100 degrees C for 15 min. The activity was maximum at pH 6.0 and was completely inactivate at pH 3.0 and pH 9.0, and rapidly reduced after storage over 3 d at 4 degrees C. The anti-LT serum could completely inhibited the activity of CE.
%K Campylobacter jejuni
%K Cytotonic enterotoxin
%K Physicochemical vroverties<br>空肠弯曲菌
%K 细胞紧张性肠毒素
%K 理化特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=30B0520C6710B62C&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=CA4FD0336C81A37A&sid=E203FB1A272C9DD2&eid=656F8C8401D91023&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=15