%0 Journal Article
%T Recombinant Fowlpox Virus Expressing F of Newcastle Disease Virus and HA of Subtype H9N2 of Avian Influenza Virus<br>共表达新城疫病毒F基因和H9亚型禽流感病毒HA基因的重组鸡痘病毒
%A WEI Dong-Ping
%A LIU Yu-Liang
%A SHAO Wei-Xing
%A LU Jian-Hong
%A LIU Xiu-Fan
%A <br>韦栋平
%A 刘玉良
%A 邵卫星
%A 卢建红
%A 刘秀梵
%J 微生物学报
%D 2004
%I 
%X The fusion protein (F) gene of Newcastle disease virus strain F48E8 was obtained by PCR and its sequence was determined. To form recombinant plasmid p7SHF, the F gene in pGEM-TF and the hemagglutinin (HA) gene of AIV, A/Chicken/China/F/1998(H9N2) in pUCHA were cut and inserted into the randomly selected nonessential regions of the fowlpox virus (FPV) under the control of fowlpox virus promoter P E/L and the synthetic promoter Ps respectively. Then the P11-LacZ reporter gene from pppG18 was cloned into p7SHF resulting in recombinant transferring vector pFPVHF, which was transfected into chicken embryo fibroblast (CEF) monolayers pre-infected with Chinese vaccine strain 282E4 of FPV to generate recombinant fowlpox virus co-expressing HA and F (rFPV-HA-F). By selection of blue plaques on the CEF overlaid with agar containing X-gal, rFPV-HF-F was screened and purified. PCR and southern blot assays indicated that the F and HA genes had been inserted into the genomic DNA of FPV. Subsequently expression of F and HA in CEF infected with rFPV-HA-F was confirmed by indirect immunofluorescence assay.
%K Newcastle disease virus strain F48E8
%K H9 subtype avian influenza virus
%K Recombinant fowlpox virus
%K Fusion protein
%K Hemagglutinin<br>新城疫病毒
%K H9亚型禽流感病毒
%K 重组鸡痘病毒
%K 融合蛋白
%K 血凝素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=0791723634D78A1C&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=38B194292C032A66&sid=11CEECA6DA9E4AC5&eid=211C7E02AC474301&journal_id=0001-6209&journal_name=微生物学报&referenced_num=7&reference_num=14