%0 Journal Article
%T Production of Monoclonal Antibodies to Tomato Mosaic Virus and Application in Virus Detection<br>番茄花叶病毒单克隆抗体的制备及检测应用
%A Yu Cui Wu Jianxiang
%A Zhou Xueping
%A <br>于翠
%A 吴建祥
%A 周雪平
%J 微生物学报
%D 2002
%I 
%X Four hybridoma cell lines secreting monoclonal antibodies(MAbs) against Tomato mosaic virus(ToMV) were produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BAL B/c immunized by the ToMV particle. The four MAbs could specifically react with ToMV, and the MAbs from two cell lines can react with ToMV and TMV simultaneously. The titres of ascitic fluids of four MAbs ranged from 1:32,000 to 1:1,024,000 with ELISA, and the sensitivity for detection virus from the plant sap reached over 1:2000 dilution. The MAbs didn't crossreact with other plant viruses. The result of Western-blot showed that two MAbs can react with the 17.6 kD ToMV coat protein submit specifically, while the other two MAbs can not react with it, they are supposed to against conformational determinants of ToMV CP.
%K Tomato mosaic virus
%K Tobacco mosaic virus
%K Monoclonal antibodies
%K TAS
%K ELISA<br>番茄花叶病毒，
%K 烟草花叶病毒，
%K 单克隆抗体，
%K TASELISA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=7BD7C707D2780EB4&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=FB36B1C076A263FA&eid=D0E8F9CBDBE0070C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=18&reference_num=13