%0 Journal Article
%T Expression of SpltMNPV p49 Gene in Escherichia coli and Determination of The Time Course of Its Expression in Insect Cells<br>斜纹夜蛾核多角体病毒p49基因在大肠杆菌中的表达及其在昆虫细胞中的表达时相分析
%A ZENG Mei-Zhen
%A WANG Jin-Wen
%A LI Zhen
%A LONG Qing-Xin
%A WANG Xun-Zhang
%A <br>曾美珍
%A 王瑾雯
%A 李镇
%A 龙綮新
%A 王珣章
%J 微生物学报
%D 2004
%I 
%X The apoptotic suppressor p49 gene was amplified by PCR from the genome of Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) and cloned into the pMD18-T vector. Sequence analysis verified the cloned fragment. The gene was then inserted into thioredoxin fusion expression vector pThioHis C and expressed stably in E.coli BL21(DE3) by induction with IPTG. The expression products were about 30% of the total bacteria proteins and exsisted as inclusion bodies. Specific antibodies with a high titre of over 1:10000 were obtained by immunizing rabbits with the purified fusion protein and was used to determine the time course of P49 expression in SpltMNPV-infected Sl cells. The results showed that P49 expression startups within 3 hours post infection and remains low level during the whole course of infection.
%K SpltMNPV
%K p49
%K Anti-apoptosis
%K Polyclonal antibody
%K Time course of expression<br>SpltMNPV，p49，凋亡抑制，多克隆抗体，表达时相
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B87499C248703BF9&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=38B194292C032A66&sid=AF507FDD66D991DA&eid=88D36036CFF69B3C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=11