%0 Journal Article
%T Cloning, Expression and Deletion of Ademosine Deaminase Gene in Escherica coli<br>大肠杆菌腺苷脱氨酶基因的克隆、表达与缺失
%A Qian Hongliang
%A Zeng Yang
%A Ye Jiang
%A Zhang Huizhan
%A <br>钱红亮
%A 曾洋
%A 叶江
%A 张惠展
%J 微生物学报
%D 2002
%I 
%X Based on the principles of metabolic engineering, a new pathway of transforming adenosine to ATP is attempted to be built in the nucleotides metabolism of %E.coli %. Deletion of adenosine deaminase gene ( %add %) is to be needed. The %add % gene from %E.coli % MC4100 was cloned by constructing gene library and expressed through constructing two plasmids pBD1 adn pBD2. In order to get the %add %-defective strain, a 5 ^2kb DNA sequence including fragmental %add % gene and marker gene %Km +r % was made and transferred into JM83, MC4100 and BL21(DE3). The genetic stability and DNA hybridization experiment proved that two %add %-defective strain J1 and J2 come from JM83 were found. The enzyme assays indicated that strain J1 lost the adenosine deaminase activity and pBD1/JM83 showed higher adenosine deaminase activity than pUC18/JM83.
%K Adenosine deaminase
%K ATP
%K Gene deletion
%K Pathway engineering<br>腺苷脱氨酶，
%K ATP，
%K 基因缺失，
%K 途径工程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3E7DB63DE8D0A245&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=B31275AF3241DB2D&sid=710C005323C0774A&eid=15F713CDE16A589C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=9