%0 Journal Article
%T TNT-degrading enzyme of Citrobacter freundii and its regulation by carbon and nitrogen source<br>弗氏柠檬酸细菌TNT降解酶及其调节
%A W Z Li
%A Y X Yang
%A H F Yang
%A <br>李文忠
%A 杨彦希
%A 杨惠芳
%J 微生物学报
%D 1989
%I 
%X It was detected that both NAD(P)H-linked reductase (TNT-Red) and NAD(P)+-linked TNT dehydrogenase (TNT-Deh) were present in TNT-degrading enzymes of Citrobacter freundii simultaneously. The time course of formation for the enzymes and the actions of coenzymes in enzymatic reaction of TNT have been studied. The effects of varied carbon and nitrogen sources on the regulation of the enzymes were different clearly. When the concentration of NH4Cl or urea in culture medium was more than 0.1 mol/L, the formation of both TNT reductase and TNT dehydrogenase was promoted. However, the formation of these enzymes was inhibited by KNO3 in culture. The production of both reductase and dehydrogenase was promoted by glucose. The sodium citrate was able to help the formation of the TNT dehydrogenase. The activity of the TNT reductase was increased when the concentration of sodium citrate was less than 0.5%, but when it was more than 0.5%, the activity of this enzyme was decreased rapidly.
%K 弗氏柠檬酸细菌
%K 需NAD(P)H的TNT还原酶
%K 需NAD(P)+的TNT脱氢酶：酶调节
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9D4495AC2B00B56E63CBF044232C2D29&yid=1833A6AA51F779C1&vid=771469D9D58C34FF&iid=0B39A22176CE99FB&sid=7555FB9CC973F695&eid=AE09EACBCD1B2A13&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=4