%0 Journal Article
%T Mutated by iron implantation for screening high L-arginine production strains using a plate coloration method by auxotroph strain<br>结合缺陷型菌株显色法采用离子束诱变筛选高产L-精氨酸突变株
%A YANG Juan
%A RAO Zhi-Ming
%A XU Mei-Juan
%A XIA Hai-Feng
%A DOU Wen-Fang
%A XU Zheng-Hong
%A <br>杨娟
%A 饶志明
%A 徐美娟
%A 夏海锋
%A 窦文芳
%A 许正宏
%J 微生物学通报
%D 2011
%I 
%X A plate coloration method by auxotroph strain for screening high L-arginine production mutants quickly and efficiently was established. Coynebacterum crenatum SYPA5-5 which is an L-arginine producer was mutated by N+ ion implantation with an energy level of 10 keV. Combined with the directed screening method and fermentation in flasks, a mutant C. crenatum SYPA5-5-36 with a higher L-arginine production and stability was selected. The yield of L-arginine produced by this mutant could reach 35.85 g/L which was 19.5% higher than that of the original strain. Therefore, the plate coloration method by auxotroph strain could be applied to screening high L-arginine production mutants quickly and efficiently.
%K L-arginine
%K Ion implantation
%K Coynebacterum crenatum
%K Auxotroph
%K Screening<br>L-精氨酸
%K 离子束注入
%K 钝齿棒杆菌
%K 营养缺陷型
%K 筛选
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=B5B552B3CA340749C17D4BC8F3725C1D&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=59906B3B2830C2C5&sid=5A8DDE4CFC853172&eid=06AAA405D95B59CF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=19