%0 Journal Article
%T A STUDY ON DPPH FREE-RADICAL SCAVENGERS FROM XYLARIA NIGRIPES<br>黑柄炭角菌产生的DPPH自由基捕捉成分
%A Wu Genfu
%A <br>吴根福
%J 微生物学报
%D 2001
%I 
%X The DPPH free-radical scavengers from the fungal Chinese medicine 'Wulingshen' (Xylaria nigripes) were studied. After separation using silica gel colunm chromatography, MPLC and HPLC, 20 DPPH free-radical scavengers with purity higher than 85% and yield more than 2 mg were screened, in which compound B4-16 had relatively higher yield and stronger free radical scavenging activity. The formula of compound B4-16 was determined to be C10H10O4 (MW: 194) based on its FAB-MS, 1H-NMR, 13C-NMR, 1H-13C HMBC, IR spectra, and its structure was elucidated. It was identical with 5,8-dihydroxy-3-methyl-3,4-dihydroisocoumarin. At the concentration of 20 mumol/L, its DPPH free\|radical scavenging activity was as 1.67 times as that of vitamin C or 2.10 times as that of Vitamin E.
%K Xylaria nigripes
%K Free\|radical scavenger
%K Chemical structure<br>黑柄炭角菌
%K 自由基捕捉成分
%K 结构鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=BF830AD66A2FC799&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=8C27CCA578E52082&eid=2E41258BCB9A7DB2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=10&reference_num=7