%0 Journal Article
%T System Error Analysis for Terminal Restriction Fragment Length Polymorphism (T-RFLP)<br>末端限制性片段长度多态性(T-RFLP)技术的系统误差分析
%A REN Yu-Jing
%A LU Xiao-Fei
%A WAN Fang-Hao
%A LIU Wan-Xue
%A <br>任玉晶
%A 芦晓飞
%A 万方浩
%A 刘万学
%J 微生物学通报
%D 2010
%I 
%X T-RFLP is a newly developed molecular technique for microbial diversity analysis. Compared with other techniques, T-RFLP has several unparalleled advantages. However, there is no report on measuring the system errors generated in T-RFLP operational process. In this study, diversity analysis of nifH gene from 4 soil samples which represented different invaded stages after Ageratina adenophora (Sprengel) invasion was chosen as a case. In order to determine possible system errors in our study, multiple analysis in which only one procedure of the whole operational processes was changed each time were conducted. Results showed that repeatability for the results of T-RFLP analysis was influenced mostly by the types of Restriction Endonucleases, followed by the PCR procedure, while capillary electrophoresis has almost no effect.
%K T-RFLP
%K System error analysis
%K Diversity analysis nifH gene<br>T-RFLP
%K 系统误差分析
%K 多样性分析nifH基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=61C4C272CBA3F0EC9D7FE75533910ECD&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=59906B3B2830C2C5&sid=5A8DDE4CFC853172&eid=4832190D43CC78F4&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0