%0 Journal Article
%T ISOLATION AND CHARACTERIZATION OF A PLASMID pTS50, WHICH ENCODES NISIN RESISTANCE DETERMINANT IN LACTOCOCCUS LACTIS TS1640<br>一个含有乳链菌肽抗性基因的乳酸乳球菌质粒pTS50的鉴定
%A Tang Sha
%A Chen Xiuzhu
%A Yang Wei
%A Chen Meiling
%A Huan Liandong
%A <br>汤莎
%A 陈秀珠
%A 杨巍
%A 陈美玲
%A 还连栋
%J 微生物学报
%D 2001
%I 
%X Three nisin-resistant Lactococcus lactis strains were screened from 197 samples of fresh milk on a selective medium (M17) supplemented with nisin, lactose and bromocresol purple, and were confirmed to have the nisin resistance determinant (nsr) by PCR amplification. Physiological and biochemical tests as well as Lactococcus lactis specific 16S rDNA sequence analysis revealed that the three strains all belong to Lactococcus lactis subsp. lactis. A large plasmid, pTS50, was identified in L. lactis subsp. lactis TS1640 which encodes resistance to nisin following electroporation of the total plasmids DNA into L. lactis MG1363. The molecular weight of plasmid pTS50 was estimated to be 47 kb by restriction analysis of BamHI, EcoRI, HindIII, NcoI, PstI and nsr was localized on a 1.9 kb EcoRI fragment by Southern hybridization.
%K Lactococcus lactis
%K Nisin resistance
%K Plasmid
%K Restriction enzyme digest
%K Southern blot<br>乳酸乳球菌
%K 乳链菌肽抗性
%K 质粒
%K 酶切分析
%K Southern杂交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3B638E2D8A27163D&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=323E8A365B085E0B&eid=1918ADDC93A85779&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=15