%0 Journal Article
%T Molecular Cloning and Amino Acid Composition Analysis of A Halophilic Thiolase Gene<br>极端嗜盐硫解酶基因的克隆和氨基酸组成分析
%A Liu Tiehan
%A Zhou Peijin
%A <br>刘铁汉
%A 周培瑾
%J 微生物学报
%D 2002
%I 
%X 5' and 3' end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP-6 was cloned and its amino acid composition was calculated. Compared with non-halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side-chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.
%K Thiolase
%K amino acid composition
%K Halophilic protein<br>硫酸酶氨基酸组成，
%K 嗜盐蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=36CDD05F0FC16082&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=DA74B62FE4348759&eid=FED44C0135DC1D9C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=7