%0 Journal Article
%T cDNA Cloning and Sequence Analysis of Genome Segment S7 of Rice Black-streaked Dwarf Virus<br>水稻黑条矮缩病毒基因组片段S7的cDNA克隆及全序列分析
%A Zhang Hengmu
%A Chen Jianping Lei Juanli Cheng Ye Xue Qingzhong
%A <br>张恒木
%A 陈剑平
%A 雷娟利
%A 程晔
%A 薛庆中
%J 微生物学报
%D 2002
%I 
%X Genome segments 7 of zhejiang and Hebei isolates of rice black-streaked dwarf virus (RBSDV) were amplified and sequenced.Segment 7 of Zhejiang isolate was consisted of 2193 nts (EMBL accession no. AJ297427) in length and that of Hebei isolate was 2190 nts (AJ297428).Both segments contained two non-overlapping open reading frames (ORFs), which encoded two polypeptides with molecular weights of 41 kD and 36 kD. These two segments shared 99% nucleotide identity, 100% and 94.4% amino acid identities of ORF1 and ORF2, shared 93.5% and 93.8% identities at nucleotide level, 98.1% (ORF1) and 96.5%/97.8% (ORF2) at amino acid level with S7 of Japanese RBSDV, and shared 85.1% and 85.3% identities at nucleotide level, 92.3% (ORF1), 85.5%/86.8% (ORF2) at amino acid level with S6 of Italian MRDV.
%K RBSDV
%K Genome segment 7
%K Sequence analysis<br>水稻黑条矮缩病毒，
%K 基因组片段S7，
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=50C0209217F9DB8D&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=0B39A22176CE99FB&sid=1E41DF9426604740&eid=334E2BB8B9A55ABB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=17