%0 Journal Article
%T GENE CLONING AND EXPRESSION OF HUMAN BACTERICIDAL/ PERMEABILITY-INCREASING PROTEIN<br>杀菌通透性增加蛋白基因的克隆及在CHO细胞中的表达
%A Xu Junjie Xu Jing Wang Haitao
%A <br>徐俊杰
%A 徐静
%A 王海涛
%J 微生物学报
%D 2001
%I 
%X The gene of human bactericidal/permeability-increasing protein (BPI) was cloned from peripheral blood lymphocytes of a normal Chinese individual.The result of sequencing showed the gene is 1452bp encoding a 27-residue signal peptide and a 456-residue matured protein, and it has six nucleotide variations compared with the sequence reported which results in 4 different amino acids.In order to get recombinant BPI, the gene was cloned into an expressing plasmid and expressed in CHO cells. The recombinant protein was purified using cation-exchange chromatography and its bioactivity was proved with bactericidal assays.
%K Bactericidal/permeability-increasing protein
%K Gene cloning
%K Expression
%K CHO cells<br>杀菌通透性增加蛋白
%K 基因克隆
%K 表达
%K CHO细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=21382A7C30D6131CC9F21EFD7406C317&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=B31275AF3241DB2D&sid=B10D796AE1B3FEBD&eid=B28C697BC3A1BA62&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=3